CENTRE FOR ENVIRONMENT, FISHERIES AND AQUACULTURE SCIENCE · Find a Tender

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Tender: eDNA Metabarcoding and High‑Throughput Sequencing of 'Beyond the Reef' Water Samples – Anguilla & Turks and Caicos

Released 5 months ago

Closed17 Jul 2026

Est. valueEstimate£15K to £35K

LocationUnited Kingdom

AgencyCENTRE FOR ENVIRONMENT, FISHERIES AND AQUACULTURE SCIENCE

SourceFind a Tender

CategoryResearch & Development

Submissions closedClosed Fri, 17 July 2026

About

Cefas is seeking a supplier to process eDNA samples from the Blue Belt Programme’s 'Beyond the Reef' survey to the Caribbean UK Overseas Territories of Anguilla, Cayman Islands and the Turks and Caicos Islands. The work requires extraction of DNA from 348 Sterivex (0.22 µm; PES) filters (triplicated per sampling station/depth; 4 L filtered each) plus 69 field negatives (417 filters total), PCR amplification of ~170 bp 12S rRNA fragments using two primer sets (MiFish‑U for teleosts and MiFish‑E for elasmobranchs), pooling and indexing of PCR products, sequencing on a suitable high‑throughput platform (e.g., NextSeq 1000 P1 XLEAP‑SBS) to an appropriate sequence depth, and delivery of demultiplexed paired‑end raw sequence data to Cefas with a high level of transparency and input into laboratory procedures to support publication. The contract is expected to start 1 September 2026 with delivery by 1 December 2026, with essential flexibility up to 1 March 2027.

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