Public Health England · Awarded contract
Awarded

Chromosomal Stability Analysis of Human iPSCs by G-Banding for Public Health England

Awarded 21 May 2019

View live tenders
Awarded21 May 2019
Value£23,000.00
RegionSouth West
CategoryHealth & Medical
Ended 5y 6mo ago2y term
Awarded 21 May 2019Expired 20 May 2021

Public Health England awarded a contract to Cell Guidance System Ltd for the assessment of chromosomal stability in human induced pluripotent stem cells (iPSCs).

The service involved receiving, handling, and processing live cells classified as Advisory Committee on Dangerous Pathogens (ACDP) Hazard Group 2, and required experience with human iPSCs, including identifying colony cultures in the log phase of growth.

The supplier used Colcemid to arrest cells in metaphase, fixed the cells with potassium chloride (KCl) solution and 3:1 methanol:acetic acid, and assessed karyology by G-banding with at least 20 G-banded metaphase spreads per sample.

A summary of the representative karyotype was provided, along with additional information if any karyotypic abnormalities were found.

The contract value was £23,000.

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