Antigen Mining for Diagnostically Relevant Serum Proteins
Defra Network eTendering Portal · Awarded contract
AwardedAntigen Mining for Diagnostically Relevant Serum Proteins
Awarded 31 Mar 2022
Awarded31 Mar 2022
Value£61,633.00
SupplierAntigen Discovery Inc
RegionUnited Kingdom
CategoryResearch & Development
Ended 3y 7mo ago12mo term
Awarded 31 Mar 2022Expired 30 Mar 2023
About
Defra Network eTendering Portal awarded a contract to Antigen Discovery Inc for antigen mining for diagnostically relevant serum.
The supplier expressed encoded recombinant proteins by in vitro transcription/translation (IVTT) reactions of 4,081 clones representing 3,873 strain H37RV proteins (99% of genome) and printed them onto custom nitrocellulose-coated microarray slides after construction of Mtb expression plasmids by PCR/recombination cloning.
The microarray slides will be validated and checked for quality by probing with a monoclonal antibody recognizing the N-terminal poly-Histidine tag and C-terminal HA tag.
The contract value is £61,633.
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